High-level secretion of correctly processed recombinant human interleukin-1 beta in Kluyveromyces lactis.

@article{Fleer1991HighlevelSO,
  title={High-level secretion of correctly processed recombinant human interleukin-1 beta in Kluyveromyces lactis.},
  author={Reinhard Fleer and Xin Chen and N. Amellal and Patrice Yeh and Agn{\`e}s Fournier and F Guinet and Nathalie Gault and Didier Faucher and F. Folliard and Hiroshi Fukuhara},
  journal={Gene},
  year={1991},
  volume={107 2},
  pages={285-95}
}
The lactose-assimilating yeast, Kluyveromyces lactis, has been developed as a microbial host for the synthesis and secretion of human proteins. Here, we report the use of multi-copy vectors based on the 2 mu-like plasmid pKD1 from Kluyveromyces drosophilarum [Chen et al., Nucleic Acids Res. 14 (1986) 4471-4481] for the secretion of recombinant human interleukin-1 beta (reIL-1 beta). High levels of reIL-1 beta were secreted into the growth medium when the structural gene was fused in-frame to a… CONTINUE READING

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