High-level secretion of biologically active recombinant human macrophage inflammatory protein-1alpha by the methylotrophic yeast Pichia pastoris.

Abstract

The human CC chemokine macrophage inflammatory protein-1alpha (MIP-1alpha) was produced at a high level in Pichia pastoris under transcriptional control of the highly inducible alcohol oxidase 1 promoter. To ensure proper folding and secretion of the recombinant polypeptide, the MIP-1alpha gene had been fused to the Saccharomyces cerevisiae alpha-factor… (More)

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@article{Maeda2000HighlevelSO, title={High-level secretion of biologically active recombinant human macrophage inflammatory protein-1alpha by the methylotrophic yeast Pichia pastoris.}, author={Yasuhiro Maeda and Roberto Kuroki and H. Suzuki and Helmut Reilaender}, journal={Protein expression and purification}, year={2000}, volume={18 1}, pages={56-63} }