High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm.

@article{Venturi2002HighLP,
  title={High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm.},
  author={Miro Venturi and Claudia Seifert and Carola Hunte},
  journal={Journal of molecular biology},
  year={2002},
  volume={315 1},
  pages={1-8}
}
The antigen-binding fragments (Fab) of antibodies are powerful tools in clinical therapy, molecular diagnostics and basic research. However, their principal applications require pure recombinant molecules in large amounts, which are challenging to obtain. Severe limitations in yield, folding and functionality are commonly encountered in bacterial production of Fab fragments. Secretion into the oxidizing periplasm generally results in low yield, whereas expression in the reducing cytoplasmic… CONTINUE READING

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