High-level production and purification of Escherichia coli N-acetylneuraminic acid aldolase (EC 4.1.3.3).

Abstract

The Escherichia coli gene which encodes N-acetylneuraminic acid aldolase was isolated by the polymerase chain reaction, cloned into the inducible expression vector pTTQ18, and overexpressed in E. coli. The high yield of aldolase was achieved through both optimum growth of cells and efficient expression of the aldolase gene (20-30% soluble cellular protein… (More)

Topics

Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.