High-level extracellular production of α-cyclodextrin glycosyltransferase with recombinant Escherichia coli BL21 (DE3).

@article{Cheng2011HighlevelEP,
  title={High-level extracellular production of α-cyclodextrin glycosyltransferase with recombinant Escherichia coli BL21 (DE3).},
  author={Jing Cheng and Dan Wu and Sheng Chen and Jian Chen and Jing Wu},
  journal={Journal of agricultural and food chemistry},
  year={2011},
  volume={59 8},
  pages={3797-802}
}
High-level production of α-cyclodextrin glycosyltransferase (CGTase) is one of the key factors in α-cyclodextrin (CD) preparation. In the present study, a fed-batch fermentation strategy for high-cell-density cultivation of Escherichia coli and the extracellular production of recombinant α-CGTase from Paenibacillus macerans JFB05-01 was established. A combined feeding strategy based on both specific growth rate before induction and the amount of glycerol residues after induction was used to… CONTINUE READING

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