High-level expression of the native barley alpha-amylase/subtilisin inhibitor in Pichia pastoris.

@article{Micheelsen2008HighlevelEO,
  title={High-level expression of the native barley alpha-amylase/subtilisin inhibitor in Pichia pastoris.},
  author={Pernille Ollendorff Micheelsen and Peter Rahbek Ostergaard and Lene Lange and Michael Skj\ot},
  journal={Journal of biotechnology},
  year={2008},
  volume={133 4},
  pages={424-32}
}
An expression system for high-level expression of the native Hordeum vulgare alpha-amylase/subtilisin inhibitor (BASI) has been developed in Pichia pastoris, using the methanol inducible alcohol oxidase 1 (AOX1) promoter. To optimize expression, two codon-optimized coding regions have been designed and expressed alongside the wild-type coding region. To ensure secretion of the native mature protein, a truncated version of the alpha mating factor secretion signal from Saccharomyces cerevisiae… CONTINUE READING
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