High-level expression of recombinant beta-galactosidases in Lactobacillus plantarum and Lactobacillus sakei using a Sakacin P-based expression system.

@article{Halbmayr2008HighlevelEO,
  title={High-level expression of recombinant beta-galactosidases in Lactobacillus plantarum and Lactobacillus sakei using a Sakacin P-based expression system.},
  author={Elisabeth Halbmayr and Geir Mathiesen and T U Nguyen and Thomas Maischberger and Clemens K. Peterbauer and Vincent G H Eijsink and Dietmar Haltrich},
  journal={Journal of agricultural and food chemistry},
  year={2008},
  volume={56 12},
  pages={4710-9}
}
This work presents the cloning and expression of the genes encoding heterodimeric beta-galactosidases from Lactobacillus reuteri L103, Lactobacillus acidophilus R22, Lactobacillus plantarum WCFS1, and Lactobacillus sakei Lb790. These enzymes consist of two subunits of approximately 73 and 35 kDa, which are encoded by two overlapping genes, lacL and lacM, respectively. We have cloned these genes into the lactobacillal expression vectors pSIP403 and pSIP409, which are based on the sakacin P… CONTINUE READING

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