High-level expression of codon optimized foot-and-mouth disease virus complex epitopes and cholera toxin B subunit chimera in Hansenula polymorpha.

@article{Song2004HighlevelEO,
  title={High-level expression of codon optimized foot-and-mouth disease virus complex epitopes and cholera toxin B subunit chimera in Hansenula polymorpha.},
  author={Houhui Song and Li Zhou and Weihuan Fang and Yong Li and Xu Wang and H. Y. Fang and Xiangdong Li and Mingyu Wu and Bingsheng Qiu},
  journal={Biochemical and biophysical research communications},
  year={2004},
  volume={315 1},
  pages={235-9}
}
A codon optimized DNA sequence coding for foot-and-mouth disease virus (FMDV) capsid protein complex epitopes of VP1 amino acid residues 21-40, 135-160, and 200-213 was genetically fused to the N-terminal end of a 6x His-tagged cholera toxin B subunit (CTB) gene with the similar synonymous codons preferred by the methylotropic yeast Hansenula polymorpha. The fusion gene was synthesized based on a polymerase chain reaction (PCR) and subsequently overexpressed in H. polymorpha. The chimeric… CONTINUE READING

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