High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae.

@article{McDonnell1991HighLE,
  title={High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae.},
  author={Donald P McDonnell and Zafar Nawaz and C L Densmore and Nancy L. Weigel and Thinh A Pham and James H. Clark and Bert W. O'malley},
  journal={The Journal of steroid biochemistry and molecular biology},
  year={1991},
  volume={39 3},
  pages={291-7}
}
Biochemical over-expression of the human estrogen receptor was achieved using a Saccharomyces cerevisiae expression system. The receptor was produced as a novel ubiquitin fusion protein. This fusion protein is short lived in the cell and is processed to produce unfused receptor shortly after folding. Conventional high copy expression plasmids produced receptor to about 0.04% of the total soluble protein. By incorporating a defective leu2 allele into these vectors, an additional 5-fold increase… CONTINUE READING