High-level expression of a truncated wall-associated protein A from the dental cariogenic Streptococcus mutans.

@article{Yoder2000HighlevelEO,
  title={High-level expression of a truncated wall-associated protein A from the dental cariogenic Streptococcus mutans.},
  author={Sean C Yoder and Chike Cao and Kenneth E Ugen and My Lien Dao},
  journal={DNA and cell biology},
  year={2000},
  volume={19 7},
  pages={401-8}
}
Streptococcus mutans plays a primary role in the formation of dental caries. Previously, in our laboratory, an S. mutans genomic library was prepared, and the wapA gene was cloned into the shuttle vector, pSA4/4B2. To generate overexpression of wapA and to facilitate efficient purification of the WapA protein for use as an immunogen, an expression vector with the strong tac promoter was used. In order to answer questions regarding the optimization of solubility and expression based on gene size… CONTINUE READING