High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C.

@article{Velander1992HighlevelEO,
  title={High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C.},
  author={William H. Velander and Jennifer L. Johnson and Raymond L. Page and Christopher G. Russell and Anuradha Subramanian and T. D. Wilkins and Francis C. Gwazdauskas and C. W. Pittius and William N. Drohan},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 24},
  pages={12003-7}
}
Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 micrograms/ml per hr in… CONTINUE READING

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