High-level expression of a biologically active human interleukin-6 mutein.

  title={High-level expression of a biologically active human interleukin-6 mutein.},
  author={S M Skelly and Charles Tackney and Daniel J. Hicklin and Theresa Tamkins and Naomi Goldstein and Harlan W. Waksal and S Dagan},
  journal={Journal of biotechnology},
  volume={34 1},
We have constructed two different muteins of interleukin-6 (IL-6) which were expressed in Escherichia coli. Both muteins lack the first 22 N-terminal amino acids of native IL-6 and lack one or the other of the two naturally occurring pairs of cysteines at either position 45 and 51 or position 74 and 84 of IL-6. We found that there was a dramatic increase in the level of IL-6 produced from each mutein clone, compared to the level produced by the wild-type IL-6 clone. We also observed that the… CONTINUE READING

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