High-level expression and purification of soluble bioactive recombinant human heparin-binding epidermal growth factor in Escherichia coli.

Abstract

Heparin-binding epidermal growth factor (HB-EGF) is a member of highly conserved superfamily of proteins that has potential mitogenic activity and stimulates differentiation and migration of various cell types. Since HB-EGF has three intra-molecular disulfide bonds, a high expression pattern of active HB-EGF in an E. coli expression system was not… (More)
DOI: 10.1002/cbin.10454

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Cite this paper

@article{Khalili2015HighlevelEA, title={High-level expression and purification of soluble bioactive recombinant human heparin-binding epidermal growth factor in Escherichia coli.}, author={Mostafa Khalili and Mohammad Reza Soleyman and Maryam Baazm and Cordian Beyer}, journal={Cell biology international}, year={2015}, volume={39 7}, pages={858-64} }