High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110.

@article{Everett1991HighLE,
  title={High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110.},
  author={Roger D. Everett and Anne Orr and Mark Elliott},
  journal={Nucleic acids research},
  year={1991},
  volume={19 22},
  pages={6155-61}
}
Herpes simplex virus type 1 (HSV-1) encodes five immediate early (IE) polypeptides. This paper reports the construction of a baculovirus vector which expresses large amounts of Vmw110, the product of IE gene 1. The expressed protein has been purified to near homogeneity and has a mobility on SDS polyacrylamide gels identical to that of Vmw110 produced during HSV-1 infection. Characterisation of its properties indicated that it forms dimers and perhaps higher order oligomers in solution and that… CONTINUE READING

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