High-level expression and purification of Cys-loop ligand-gated ion channels in a tetracycline-inducible stable mammalian cell line: GABAA and serotonin receptors.

@article{Dostlov2010HighlevelEA,
  title={High-level expression and purification of Cys-loop ligand-gated ion channels in a tetracycline-inducible stable mammalian cell line: GABAA and serotonin receptors.},
  author={Zuzana Dost{\'a}lov{\'a} and Aiping Liu and Xiaojuan Zhou and Sarah Linda Farmer and Eileen Susan Krenzel and Enrique Armas Ar{\'e}valo and Rooma Desai and Paula Leslie Feinberg-Zadek and Paul A. Davies and Innocent H. Yamodo and Stuart A. Forman and Keith W Miller},
  journal={Protein science : a publication of the Protein Society},
  year={2010},
  volume={19 9},
  pages={
          1728-38
        }
}
The human neuronal Cys-loop ligand-gated ion channel superfamily of ion channels are important determinants of human behavior and the target of many drugs. It is essential for their structural characterization to achieve high-level expression in a functional state. The aim of this work was to establish stable mammalian cell lines that enable high-level heterologous production of pure receptors in a state that supports agonist-induced allosteric conformational changes. In a tetracycline… CONTINUE READING
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