High-level expression and molecular cloning of genes encoding Candida tropicalis peroxisomal proteins.

@article{Kamiryo1984HighlevelEA,
  title={High-level expression and molecular cloning of genes encoding Candida tropicalis peroxisomal proteins.},
  author={Tatsuyuki Kamiryo and Koei Okazaki},
  journal={Molecular and cellular biology},
  year={1984},
  volume={4 10},
  pages={2136-41}
}
The development of peroxisomes in the cells of Candida tropicalis grown on oleic acid was accompanied by a markedly high expression of peroxisomal proteins. On the basis of this finding, the nuclear DNA library of this yeast was screened by differential hybridization, and 102 clones of oleic acid-inducible sequences were isolated. Seven coding regions were found to form clusters in three stretches of the genomic DNA. Five of the regions were identified as genes for peroxisomal polypeptides… CONTINUE READING