High-level expression, purification and characterisation of porcine β-defensin 2 in Pichia pastoris and its potential as a cost-efficient growth promoter in porcine feed

@article{Peng2014HighlevelEP,
  title={High-level expression, purification and characterisation of porcine β-defensin 2 in Pichia pastoris and its potential as a cost-efficient growth promoter in porcine feed},
  author={Zixin Peng and Anru Wang and Qiuyue Feng and Zhaoyue Wang and Iskra Vitanova Ivanova and Xiuping He and Borun Zhang and Weiping Song},
  journal={Applied Microbiology and Biotechnology},
  year={2014},
  volume={98},
  pages={5487-5497}
}
Porcine β-defensin 2 (pBD2), a recently discovered porcine defensin that is produced by the intestine, exerts antimicrobial activities and innate immune effects that are linked to intestinal diseases in pigs. Here, we report a codon-optimised protein corresponding to mature pBD2 cDNA that was expressed and purified in Pichia pastoris yeast. The highest amount of secreted protein (3,694.0 mg/L) was reached 144 h into a 150-h induction during high-density cultivation. Precipitation followed by… CONTINUE READING
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