High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.

@article{Takagi2007HighIC,
  title={High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.},
  author={Mutsumi Takagi and Yousuke Umetsu and Masashi Fujiwara and Shigeyuki Wakitani},
  journal={Journal of bioscience and bioengineering},
  year={2007},
  volume={103 1},
  pages={
          98-100
        }
}
The effects of the density of human mesenchymal stem cells (MSCs) on their differentiation to chondrocytes in a differentiation medium supplemented with dexamethasone, TGFbeta3, and IGF-1 were investigated for the regenerative therapy of cartilage. The increase in the initial density of MSCs from 0.05 x 10(4) to 0.9 x 10(4) cells/cm(2) accelerated the increase in the expression level of aggrecan mRNA during the differentiation culture for 7 d. The conditioned medium harvested at 7 d from the… 
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47 Citations
Background Citations
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Methods Citations
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Results Citations
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47 Citations

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Citation Type

New Approach for Differentiation of Bone Marrow Mesenchymal Stem Cells Toward Chondrocyte Cells With Overexpression of MicroRNA-140
TLDR
A new approach and technique that may be applied for differentiation of BMSCs to chondrocyte instead of stimulation with TGF&bgr;3 is described and implies that miR-140 is a potent chondrogenic differentiation inducer for B MSCs.
Impact of Cell Density on Differentiation Efficiency of Rat Adipose-derived Stem Cells into Schwann-like Cells
TLDR
The results suggest that the higher differentiation rate of ASCs to SC-like cells can be obtained at initial cell density of 8×103 cells/cm2, possibly via increased cell-cell interaction and cell density-dependent influence of glial growth factors.
Chondrogenesis of human bone marrow mesenchymal stem cells in fibrin-polyurethane composites.
TLDR
The scaffold composite culture system shows chondrogenesis of hMSCs comparable with that of pellet culture, and is founded a basic system for cartilage neo-tissue formation in vitro.
Comparison between High Cell-Density Culture Systems for Chondrogenic Differentiation and Articular Cartilage Reconstruction of Human Mesenchymal Stem Cells: A Literature Review
TLDR
There will be a review about the methods of chondrogenic differentiation of hMSCs that utilized in in vitro and in vivo chondogenic differentiation of stem cells for treatment of osteoarthritis and the micromass culture system is the best tool for in vitro chondrogensic differentiation studies.
Heterogeneous differentiation of human mesenchymal stem cells in response to extended culture in extracellular matrices.
TLDR
It is shown that ECMs independently trigger differentiation of human adult MSCs and that differentiation in this context can be guided down multiple lineages using the same ECM stimulus.
Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems
TLDR
In micromass culture, the induced-cartilage tissues were larger, more homogenous and enriched in cartilage-specific collagen II but the fibrocartilage-like feature, collagen I, and hypertrophic chondrocytefeature, collagen X, were markedly decreased compared to those in pellet culture.
Effect of dexamethasone supplementation on chondrogenesis of equine mesenchymal stem cells.
OBJECTIVE To determine whether expansion of equine mesenchymal stem cells (MSCs) by use of fibroblast growth factor-2 (FGF-2) prior to supplementation with dexamethasone during the chondrogenic…
Enhanced chondrogenesis differentiation of human induced pluripotent stem cells by MicroRNA-140 and transforming growth factor beta 3 (TGFβ3).
Regulation of nestin expression by thrombin and cell density in cultures of bone mesenchymal stem cells and radial glial cells
TLDR
It is demonstrated that thrombin stimulates the growth of radial glial cells and mesenchymal stem cells (MSCs) in a dose-dependent manner and that nestin expression by MSCs and RG is regulated in opposite manner by Thrombin in vitro.
Identification and In Vitro Expansion of Buccal Epithelial Cells
TLDR
It is demonstrated that seeding density has a profound effect on the proliferation and differentiation of BME stem cells in vitro, and this is relevant to downstream cell therapy applications.
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References

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TLDR
The sequential two-step differentiation cultivation, during which the cells were cultivated in 3D for 1 week after the conventional two-dimensional (2D) culture, could markedly accelerate the expression of aggrecan mRNA compared with the 3D cultivation for 2 week.
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TLDR
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