High homology is not required at the site of strand invasion during recombinational double-strand break repair in mammalian chromosomes.

Abstract

We investigated the impact of sequence divergence on DNA double-strand break (DSB) repair occurring via recombination in cultured thymidine kinase deficient mouse fibroblasts. We stably transfected cells with a DNA construct harboring a herpes thymidine kinase (tk) gene (the "recipient") rendered nonfunctional by insertion of an oligonucleotide containing… (More)
DOI: 10.1016/j.dnarep.2017.10.006

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