High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of Pst isolate CY32, obtained using a 'fosmid-to-fosmid' strategy, to better understand its race evolution and pathogenesis. The Pst genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pathogens, Pst has a more diverse gene composition and more genes encoding secreted proteins. Re-sequencing analysis indicates significant genetic variation among six isolates collected from different continents. Approximately 35% of SNPs are in the coding sequence regions, and half of them are non-synonymous. High genetic diversity in Pst suggests that sexual reproduction has an important role in the origin of different regional races. Our results show the effectiveness of the 'fosmid-to-fosmid' strategy for sequencing dikaryotic genomes and the feasibility of genome analysis to understand race evolution in Pst and other obligate pathogens.

DOI: 10.1038/ncomms3673

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@inproceedings{Zheng2013HighGH, title={High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus}, author={Wenming Zheng and Lili Huang and Jinqun Huang and Xiaojie Wang and Xianming Chen and Jie Zhao and Jun Guo and Hua Zhuang and Chuangzhao Qiu and Jie Liu and Huiquan Liu and Xueling Huang and Guoliang Pei and Gangming Zhan and Chunlei Tang and Yulin Cheng and Minjie Liu and Jinshan Zhang and Zhongtao Zhao and Shijie Zhang and Qingmei Han and Dejun Han and Hongchang Zhang and Jing Zhao and Xiaoning Gao and Jianfeng Wang and Peixiang Ni and Wei Dong and Linfeng Yang and Huanming Yang and Jin-Rong Xu and Gengyun Zhang and Zhensheng Kang}, booktitle={Nature communications}, year={2013} }