High genetic variability in non-aflatoxigenic A. flavus strains by using Quadruplex PCR-based assay.

  title={High genetic variability in non-aflatoxigenic A. flavus strains by using Quadruplex PCR-based assay.},
  author={G. Criseo and Cosimo Racco and O. Romeo},
  journal={International journal of food microbiology},
  volume={125 3},
  • G. Criseo, Cosimo Racco, O. Romeo
  • Published 2008
  • Biology, Medicine
  • International journal of food microbiology
  • Aflatoxigenic Aspergillus flavus isolates always show, by using a multiplex PCR-system, four DNA fragments specific for aflR, nor-1, ver-1, and omt-A genes. Non-aflatoxigenic A. flavus strains give variable DNA banding pattern lacking one, two, three or four of these genes. Recently, it has been found and reported that some aflatoxin non-producing A. flavus strains show a complete set of genes. Because less is known about the incidence of structural genes aflR, nor-1, ver-1 and omt-A in… CONTINUE READING
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