High genetic variability in non-aflatoxigenic A. flavus strains by using Quadruplex PCR-based assay.

@article{Criseo2008HighGV,
  title={High genetic variability in non-aflatoxigenic A. flavus strains by using Quadruplex PCR-based assay.},
  author={G. Criseo and Cosimo Racco and O. Romeo},
  journal={International journal of food microbiology},
  year={2008},
  volume={125 3},
  pages={
          341-3
        }
}
  • G. Criseo, Cosimo Racco, O. Romeo
  • Published 2008
  • Biology, Medicine
  • International journal of food microbiology
    • Aflatoxigenic Aspergillus flavus isolates always show, by using a multiplex PCR-system, four DNA fragments specific for aflR, nor-1, ver-1, and omt-A genes. Non-aflatoxigenic A. flavus strains give variable DNA banding pattern lacking one, two, three or four of these genes. Recently, it has been found and reported that some aflatoxin non-producing A. flavus strains show a complete set of genes. Because less is known about the incidence of structural genes aflR, nor-1, ver-1 and omt-A in… CONTINUE READING
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    References

    SHOWING 1-10 OF 15 REFERENCES
    Multiplex polymerase chain reaction for the detection of potential aflatoxin and sterigmatocystin producing fungi
    • 173
    • PDF
    Sequence breakpoints in the aflatoxin biosynthesis gene cluster and flanking regions in nonaflatoxigenic Aspergillus flavus isolates.
    • 200
    Detection of aflatoxigenic molds in grains by PCR.
    • 190
    • Highly Influential
    • PDF
    Aflatoxigenicity in Aspergillus: molecular genetics, phylogenetic relationships and evolutionary implications
    • 98
    Detection of transcripts of the aflatoxin genes aflD, aflO, and aflP by reverse transcription-polymerase chain reaction allows differentiation of aflatoxin-producing and non-producing isolates of Aspergillus flavus and Aspergillus parasiticus.
    • 130
    Detection of aflatoxigenic fungi in selected food commodities by PCR
    • 66
    Detection of aflatoxinogenic fungi in figs by a PCR reaction.
    • 116
    Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real-time PCR, and its correlation to the cfu of Aspergillus flavus in foods.
    • 140
    PCR-restriction fragment length analysis of aflR gene for differentiation and detection of Aspergillus flavus and Aspergillus parasiticus in maize.
    • 42
    Detection of aflatoxigenic fungi in feeds using the PCR method
    • 21