High frequency transformation of Cryptococcus neoformans and Cryptococcus gattii by Agrobacterium tumefaciens.

Abstract

Cryptococcus neoformans and Cryptococcus gattii are the caus-ative agents of cryptococcal meningoencephalitis and are amenable to genetic manipulations, making them important models of pathogenic fungi. To improve the efficiency of Agrobacterium tumefaciens mediated transformation (ATMT) in C. neoformans, we optimized various co-cultivation conditions… (More)

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