High frequency somatic embryogenesis and plantlet regeneration from hypocotyl protoplast cultures of Brassica napus

@article{Parihar2004HighFS,
  title={High frequency somatic embryogenesis and plantlet regeneration from hypocotyl protoplast cultures of Brassica napus},
  author={Dwarkesh S. Parihar and Satish Chandra Maheshwari and Paramjit Khurana},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={42},
  pages={113-115}
}
A simple protocol has been developed for high frequency protoplast regeneration via somatic embryogenesis in B. napus. Protoplasts isolated from hypocotyl tissue of 8–12 day old seedlings of Brassica napus ISN706 (AACC) when cultured in KM(A) medium resulted in divisions with a, frequency ranging from 30–35%. Regeneration of plantlets was possible by both organogenesis and embryogenesis. Nearly 80% of the call transferred on to MS medium supplemented with 5.0 mg l-1 2iP, 0.1 mg l-1 NAA, 0.001… CONTINUE READING

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