High frequency shoot regeneration of Sterculia urens Roxb. an endangered tree species through cotyledonary node cultures

  title={High frequency shoot regeneration of Sterculia urens Roxb. an endangered tree species through cotyledonary node cultures},
  author={Town Mohammad Hussain and Thummala Chandrasekhar and Ghanta Rama Gopal},
  journal={African Journal of Biotechnology},
A protocol is described for rapid and large scale propagation of an endangered, commercially and medicinally important tree species, Sterculia urens, by in vitro culture of cotyledonary nodes from 15 days old seedlings. Of the four different cytokinins (thidiazuron, isopentenyladenine, zeatin and adenine sulphate) evaluated as supplements to Murashige and Skoog medium (1962), thidiazuron at an optimal concentration of 2.27 M was most effective in inducing bud break (83.0%). Although, multiple… 

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