High-frequency shoot regeneration from leaf explants through organogenesis in bitter melon (Momordica charantia L.)

@article{Thiruvengadam2010HighfrequencySR,
  title={High-frequency shoot regeneration from leaf explants through organogenesis in bitter melon (Momordica charantia L.)},
  author={Muthu Thiruvengadam and Kaliyaperumal Rekha and C. Yang and Narayanasamypillai Jayabalan and Ill Min Chung},
  journal={Plant Biotechnology Reports},
  year={2010},
  volume={4},
  pages={321-328}
}
An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g l−1 sucrose, 2.2 g l−1 Gelrite, and 7.7 μM naphthalene acetic… CONTINUE READING
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