High-frequency secondary mutations after suicide-driven allelic exchange mutagenesis in extraintestinal pathogenic Escherichia coli.

@article{Johnson2003HighfrequencySM,
  title={High-frequency secondary mutations after suicide-driven allelic exchange mutagenesis in extraintestinal pathogenic Escherichia coli.},
  author={James R. Johnson and Hank A. Lockman and Krista L Owens and Srdjan Jela{\vc}i{\'c} and Phillip I Tarr},
  journal={Journal of bacteriology},
  year={2003},
  volume={185 17},
  pages={5301-5}
}
Frequent unintended secondary mutations occurred in extraintestinal pathogenic Escherichia coli strains CP9, CFT073, and RS218 during suicide plasmid-mediated, putatively specific deletions of hlyA, papG allele III, and iha. Pulsed-field gel electrophoresis and PCR analyses demonstrated genomic alterations and/or unintended loss of defined virulence genes (papG, the F7-2 papA allele, iutA, sat, hlyD, and cnf). Caution is warranted when attributing the observed phenotypic changes to the intended… CONTINUE READING

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