High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene

@inproceedings{SauHighfrequencyPT,
  title={High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene},
  author={Andrea Sau}
}
We report here a 100-fold increased frequency of plastid transformation in tobacco by selection for a chimeric aadA gene encoding aminoglycoside 3"-adenylyltransferase, as compared with that obtained with mutant 16S rRNA genes. Expression of aad4 confers resistance to spectinomycin and streptomycin. In transforming plasmid pZS197, a chimeric aadA is cloned between rbcL and open reading frame ORF512 plastid gene sequences. Selection was for spectinomycin resistance after biolistic delivery of… CONTINUE READING
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