High frequency plant regeneration via somatic embryogenesis in cell suspension cultures of cowpea, Vigna unguiculata (L.) Walp.

@article{Anand2000HighFP,
  title={High frequency plant regeneration via somatic embryogenesis in cell suspension cultures of cowpea, Vigna unguiculata (L.) Walp.},
  author={Ranjith P. Anand and A. S. Ganapathi and Vasudevan Ramesh Anbazhagan and Gnanamoorthi Vengadesan and Natesan Selvaraj},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2000},
  volume={36},
  pages={475-480}
}
Embryogenic callus was induced from primary leaves of Vigna unguiculata (L.) Walp. in MS medium (Murashige and Skoog, 1962) containing 2,4-dichlorophenoxyacetic acid (2,4-D). Greenish-white, friable embryogenic calluses were used to establish suspension cultures. A shaking speed of 90 rpm and 0.4 ml packed cell volume per 25 ml medium were found to be optimal for maintaining suspension cultures. Globular, heart-shaped and torpedo-shaped embryos were developed in suspension culture containing 4… CONTINUE READING
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