High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

@inproceedings{Fu2013HighFO,
  title={High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells},
  author={Yanfang Fu and Jennifer A. Foden and Cyd Khayter and Morgan L. Maeder and Deepak Reyon and J. Keith Joung and Jeffry D. Sander},
  booktitle={Nature Biotechnology},
  year={2013}
}
Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases (RGNs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we use a human cell–based reporter assay to characterize off-target cleavage of CRISPR-associated (Cas)9-based RGNs. We find that single and double mismatches are tolerated to varying degrees depending on their position along the guide RNA (gRNA)-DNA interface. We also readily detected off-target alterations induced by… CONTINUE READING
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