High-frequency generation of viable mice from engineered bi-maternal embryos

@article{Kawahara2007HighfrequencyGO,
  title={High-frequency generation of viable mice from engineered bi-maternal embryos},
  author={Manabu Kawahara and Qiong Wu and Nozomi Takahashi and Shinnosuke Morita and Kaori Yamada and Mitsuteru Ito and Anne C. Ferguson-Smith and Tomohiro Kono},
  journal={Nature Biotechnology},
  year={2007},
  volume={25},
  pages={1045-1050}
}
Mammalian development to adulthood typically requires both maternal and paternal genomes, because genomic imprinting places stringent limitations on mammalian development, strictly precluding parthenogenesis. Here we report the generation of bi-maternal embryos that develop at a high success rate equivalent to the rate obtained with in vitro fertilization of normal embryos. These bi-maternal mice developed into viable and fertile female adults. The bi-maternal embryos, distinct from… Expand
Roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse ontogeny.
TLDR
The technique for generating bi-maternal mice was refined and it was found that genetic manipulations in only 2 regions--the imprinting centres of Igf2-H19 and Dlk1-Gtl2--on chromosomes 7 and 12 of the newborn pups allowed the mice to be generated at a high rate. Expand
Three paternally imprinted regions are sequentially required in prenatal and postnatal mouse development
TLDR
Consistent with the essential functions of genomic imprinting in mammalian development, loss-of-function mouse genetic studies demonstrated that a portion of the imprinted genes such as Igf2 were essential for the survival of mouse embryos when they had been knocked out. Expand
Genetic modification for bimaternal embryo development.
  • T. Kono
  • Biology, Medicine
  • Reproduction, fertility, and development
  • 2009
TLDR
Using bimaternal embryos with two sets of maternal genomes, the present paper illustrates how parental methylation imprints are an obstacle to the progression of parthenogenesis. Expand
Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi‐maternal embryos to term
TLDR
Comparison with BMEs derived from Igf2+/− ng/fg genomes suggests that bi‐allelic H19 expression might be involved in foetal development, and restoration of imprinted gene expression from two regions is reported. Expand
Protocol for the production of viable bimaternal mouse embryos
TLDR
This oocyte reconstruction system, as described in this protocol, could provide valuable guidelines for exploring the potential endowments of gametes and for conferring novel properties to them. Expand
Temporal regulation of prenatal embryonic development by paternal imprinted loci
TLDR
The results indicate the temporal regulation of paternal imprinted loci during embryonic development using androgenetic haESC (AG-haESC)-mediated semi-cloned (SC) technology. Expand
Temporal regulation of prenatal embryonic development by paternal imprinted loci
TLDR
The results indicate the temporal regulation of paternal imprinted loci during embryonic development using androgenetic haESC (AG-haESC)-mediated semi-cloned (SC) technology. Expand
Generation of Viable Male and Female Mice from Two Fathers1
In sexual species, fertilization of oocytes produces individuals with alleles derived from both parents. Here we use pluripotent stem cells derived from somatic cells to combine the haploid genomesExpand
Polyploidy of semi-cloned embryos generated from parthenogenetic haploid embryonic stem cells
TLDR
The generation of fertile semi-cloned mice is shown by injection of parthenogenetic haESCs (phaESCs) into oocytes after deletion of two differentially methylated regions (DMRs), the IG-D MR and H19-DMR. Expand
Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer
TLDR
The results suggest that there is a limitation for foetal development in the ability to reprogramme imprinted genes by repeated rounds of nuclear transfer, however, the placentas of parthenogenetic embryos can escape epigenetic regulation when developed using nuclear transfer techniques and can support foetAL development to full gestation. Expand
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