High-frequency gene targeting in Arabidopsis plants expressing the yeast RAD54 gene.

@article{Shaked2005HighfrequencyGT,
  title={High-frequency gene targeting in Arabidopsis plants expressing the yeast RAD54 gene.},
  author={Hezi Shaked and Cathy Melamed-Bessudo and Avraham A Levy},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 34},
  pages={12265-9}
}
Gene targeting, which is homologous recombination-mediated integration of an extra-chromosomal DNA segment into a chromosomal target sequence, enables the precise disruption or replacement of any gene. Despite its value as a molecular genetic tool, gene targeting remains an inefficient technology in most species. We report that expression of the yeast RAD54 gene, a member of the SWI2/SNF2 chromatin remodeling gene family, enhances gene targeting in Arabidopsis by one to two orders of magnitude… CONTINUE READING

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A N T Bi O Lo G Y Se E Co M M En Ta Ry

  • 763. Shaked
  • PNAS August
  • 2003

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