High-frequency disruption of the N-myc gene in embryonic stem and pre-B cell lines by homologous recombination.

@article{Charron1990HighfrequencyDO,
  title={High-frequency disruption of the N-myc gene in embryonic stem and pre-B cell lines by homologous recombination.},
  author={Jean Charron and Barbara A Malynn and Elisabeth J Robertson and Stephen P Goff and Frederick W Alt},
  journal={Molecular and cellular biology},
  year={1990},
  volume={10 4},
  pages={1799-804}
}
Identification of gene function has often relied on isolation of mutant cells in which expression of the gene was inactivated. Gene targeting by homologous recombination in tissue culture now may provide a technology to rapidly and directly produce such mutant mammalian cells. We demonstrate that selection of embryonic stem and pre-B cell lines for expression of a promoterless construct containing murine N-myc genomic sequences fused to a gene encoding neomycin resistance allows highly… CONTINUE READING