High frequency adventitious shoot regeneration from immature cotyledons of pea (Pisum sativum L.)

@article{zcan1992HighFA,
  title={High frequency adventitious shoot regeneration from immature cotyledons of pea (Pisum sativum L.)},
  author={Sebahattin {\"O}zcan and Mehdi Barghchi and S. Firek and John Draper},
  journal={Plant Cell Reports},
  year={1992},
  volume={11},
  pages={44-47}
}
A procedure has been developed which allows high frequency adventitious shoot regeneration from immature cotyledons of pea. Prolific shoot regeneration occurred following an initial callus growth on a Murashige and Skoog (MS) medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 4 mg/l α-naphthaleneacetic acid (NAA). Cotyledon expiants proximal to the embryonic axis had the highest regeneration potential, however, the presence of an embryonic axis inhibited adventitious shoot regeneration… CONTINUE READING