High-flow multiplexed MRM-based analysis of proteins in human plasma without depletion or enrichment.

@article{Domanski2011HighflowMM,
  title={High-flow multiplexed MRM-based analysis of proteins in human plasma without depletion or enrichment.},
  author={Dominik Domanski and Derek S Smith and Christine A. Miller and Yanan Yang and Angela M. Jackson and Gabriela V Cohen Freue and J. S. Hill and Carol E. Parker and Christoph H. Borchers},
  journal={Clinics in laboratory medicine},
  year={2011},
  volume={31 3},
  pages={371-84}
}
Jeff Whiteaker’s chapter within this issue describes the use of stable isotope standards and capture by anti-peptide antibodies (SISCAPA), an analytical technique that combines immunopurification with co-capture of isotopically-labeled standards, elution, and multiple reaction monitoring (MRM) analysis. While affinity-based enrichment may be necessary for low abundance analytes, in our laboratory we have found that, for the analysis of high-to-moderate abundance plasma proteins, it is often… CONTINUE READING
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