High-fidelity mRNA amplification for gene profiling

Abstract

The completion of the Human Genome Project has made possible the comprehensive analysis of gene expression, and cDNA microarrays are now being employed for expression analysis in cancer cell lines or excised surgical specimens. However, broader application of cDNA microarrays is limited by the amount of RNA required: 50–200 μg of total RNA (T-RNA) and 2–5… (More)
DOI: 10.1038/74546

Topics

3 Figures and Tables

Cite this paper

@article{Wang2000HighfidelityMA, title={High-fidelity mRNA amplification for gene profiling}, author={Ena Wang and Lance D. Miller and Galen A. Ohnmacht and Edison T. Liu and Francesco M. Marincola}, journal={Nature Biotechnology}, year={2000}, volume={18}, pages={457-459} }