High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase

@article{Eckert1990HighFD,
  title={High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase},
  author={K. A. Eckert and T. A. Kunkel},
  journal={Nucleic acids research},
  year={1990},
  volume={18 13},
  pages={3739-44}
}
We demonstrate that despite lacking a 3'----5' proofreading exonuclease, the Thermus aquaticus (Taq) DNA polymerase can catalyze highly accurate DNA synthesis in vitro. Under defined reaction conditions, the error rate per nucleotide polymerized at 70 degrees C can be as low as 10(-5) for base substitution errors and 10(-6) for frameshift errors. The frequency of mutations produced during a single round of DNA synthesis of the lac Z alpha gene by Taq polymerase responds to changes in dNTP… CONTINUE READING