High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts

@article{Li2005HighEO,
  title={High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts},
  author={Yin{\"u} Li and Meng Sun and Ji-xing Liu and Zongqi Yang and Zhifang Zhang and Guifang Shen},
  journal={Plant Cell Reports},
  year={2005},
  volume={25},
  pages={329-333}
}
A tobacco chloroplast expression vector, pTRVP1, containing the foot-and-mouth disease virus (FMDV) VP1 gene and the selective marker aadA gene, was constructed and transferred to tobacco by biolistic method. Three resistant lines were obtained through spectinomycin selection, and each transgenic line was subjected to a second round of spectinomycin selection. PCR and PCR southern blot analysis revealed that the VP1 gene had integrated into the chloroplast genome. Western blot and… CONTINUE READING