High-end pH-controlled delivery of glucose effectively suppresses lactate accumulation in CHO fed-batch cultures.

Abstract

A simple method for control of lactate accumulation in suspension cultures of Chinese hamster ovary (CHO) cells based on the culture's pH was developed. When glucose levels in culture reach a low level (generally below 1 mM) cells begin to take up lactic acid from the culture medium resulting in a rise in pH. A nutrient feeding method has been optimized… (More)
DOI: 10.1002/bit.23072

Topics

  • Presentations referencing similar topics