High-end pH-controlled delivery of glucose effectively suppresses lactate accumulation in CHO fed-batch cultures.


A simple method for control of lactate accumulation in suspension cultures of Chinese hamster ovary (CHO) cells based on the culture's pH was developed. When glucose levels in culture reach a low level (generally below 1 mM) cells begin to take up lactic acid from the culture medium resulting in a rise in pH. A nutrient feeding method has been optimized… (More)
DOI: 10.1002/bit.23072


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