High-efficiency transformation system for the biocontrol agents, Trichoderma spp.

@article{HerreraEstrella1990HighefficiencyTS,
  title={High-efficiency transformation system for the biocontrol agents, Trichoderma spp.},
  author={Alfredo Herrera-Estrella and Gustavo Henrique Goldman and Marc C E Van Montagu},
  journal={Molecular microbiology},
  year={1990},
  volume={4 5},
  pages={839-43}
}
We have developed an efficient transformation system based on the use of polyethylene glycol and CaCl2 for the biocontrol agents, Trichoderma spp. Transformation was obtained with the plasmid pAN7-1, carrying a bacterial hygromycin-resistance gene as a selectable marker, under the control of Aspergillus nidulans heterologous expression signals. The system described here yielded 200-800 transformants per microgram of DNA. Transformants contained several copies of the plasmid integrated into… CONTINUE READING

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