High efficiency transformation of tall fescue with Agrobacterium tumefaciens

@inproceedings{Dong2005HighET,
  title={High efficiency transformation of tall fescue with Agrobacterium tumefaciens},
  author={Shujie Dong and Rongda Qu},
  year={2005}
}
An efficient genetic transformation system for tall fescue ( Festuca arundinacea Schreb.), using Agrobacterium tumefaciens-mediated TDNA delivery, is described. Seed-derived embryogenic calli were infected with Agrobacterium tumefaciens strain EHA105 harboring plasmids pTOK47 and pCAMBIA1301. Infected calli were selected at 250 mg L 1 hyg B and the regenerated plantlets at 50 mg L . Using the protocol developed, 34% of the calli infected were hyg B resistant, and the overall plant… CONTINUE READING

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