High efficiency transformation of Schizosaccharomyces pombe pretreated with thiol compounds by electroporation.

@article{Suga2001HighET,
  title={High efficiency transformation of Schizosaccharomyces pombe pretreated with thiol compounds by electroporation.},
  author={Minoru Suga and Toyomasa Hatakeyama},
  journal={Yeast},
  year={2001},
  volume={18 11},
  pages={1015-21}
}
A highly efficient method for transformation of the fission yeast Schizosaccharomyces pombe by electroporation has been developed. Significantly higher transformation efficiency was obtained when intact cells grown in SD medium (0.67% Bacto yeast nitrogen base without amino acids, 2% glucose) were pretreated with thiol compounds before an electric pulse was applied to the cells. Among the thiol compounds tested, dithiothreitol (DTT) was the most effective for pretreatment. A high transformation… CONTINUE READING

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Transformation of Schizosaccharomyces pombe: Electroporation Procedure.

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