High-efficiency transfer of the T cell co-stimulatory molecule B7-2 to lymphoid cells using high-titer recombinant adeno-associated virus vectors.

@article{Chiorini1995HighefficiencyTO,
  title={High-efficiency transfer of the T cell co-stimulatory molecule B7-2 to lymphoid cells using high-titer recombinant adeno-associated virus vectors.},
  author={John A. Chiorini and Clemens Martin Wendtner and E Urcelay and Brian Safer and Michael J Hallek and Robert M. Kotin},
  journal={Human gene therapy},
  year={1995},
  volume={6 12},
  pages={1531-41}
}
Adeno-associated virus (AAV) is a single-stranded DNA virus that can either integrate or replicate in host cells. Production of recombinant viral particles (rAAV) requires expression of the viral structural genes and the viral inverted terminal repeats in cis. By using an SV40 replicon to amplify the structural genes, the yield of recombinant viral particles was increased 60-fold over a nonreplicating helper plasmid. The rAAV particles produced by this system have similar physical properties to… CONTINUE READING

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