High efficiency transfection of Plasmodium berghei facilitates novel selection procedures.

@article{Janse2006HighET,
  title={High efficiency transfection of Plasmodium berghei facilitates novel selection procedures.},
  author={Chris J Janse and Blandine M.D. Franke-Fayard and Gunnar R Mair and Jai Ramesar and Corinna Thiel and Sabine Engelmann and Kai Matuschewski and Geert Jan A van Gemert and Robert W. Sauerwein and Andrew P Waters},
  journal={Molecular and biochemical parasitology},
  year={2006},
  volume={145 1},
  pages={60-70}
}
The use of transfection in the study of the biology of malaria parasites has been limited due to poor transfection efficiencies (frequency of 10(-6) to 10(-9)) and a paucity of selection markers. Here, a new method of transfection, using non-viral Nucleofector technology, is described for the rodent parasite Plasmodium berghei. The transfection efficiency obtained (episomal and targeted integration into the genome) is in the range of 10(-2) to 10(-3). Such high transfection efficiency strongly… CONTINUE READING
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