High efficiency transfection of Plasmodium berghei facilitates novel selection procedures.

  title={High efficiency transfection of Plasmodium berghei facilitates novel selection procedures.},
  author={Chris J Janse and Blandine M.D. Franke-Fayard and Gunnar R Mair and Jai Ramesar and Corinna Thiel and Sabine Engelmann and Kai Matuschewski and Geert Jan A van Gemert and Robert W. Sauerwein and Andrew P Waters},
  journal={Molecular and biochemical parasitology},
  volume={145 1},
The use of transfection in the study of the biology of malaria parasites has been limited due to poor transfection efficiencies (frequency of 10(-6) to 10(-9)) and a paucity of selection markers. Here, a new method of transfection, using non-viral Nucleofector technology, is described for the rodent parasite Plasmodium berghei. The transfection efficiency obtained (episomal and targeted integration into the genome) is in the range of 10(-2) to 10(-3). Such high transfection efficiency strongly… CONTINUE READING
Highly Influential
This paper has highly influenced 10 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

From This Paper

Topics from this paper.


Publications citing this paper.
Showing 1-10 of 180 extracted citations

Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes.

Proceedings of the National Academy of Sciences of the United States of America • 2013
View 4 Excerpts
Highly Influenced

Transient transfection of purified Babesia bovis merozoites.

Experimental parasitology • 2008
View 10 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…