High efficiency reconstitution of a phencyclidine/MK-801 receptor binding site solubilized from rat forebrain membranes.

Abstract

Phencyclidine (PCP) receptors have been solubilized from rat forebrain membranes with the zwitterionic detergent 3-(3-cholamidopropyl)dimethylammonio-1-propanesulfonate. Specific binding of the potent PCP receptor ligands [3H]thienyl-phencyclidine (TCP) and [3H]MK-801 was restored by incorporating extracted membrane protein into lipid vesicles prepared from… (More)

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@article{Haring1991HighER, title={High efficiency reconstitution of a phencyclidine/MK-801 receptor binding site solubilized from rat forebrain membranes.}, author={Robin Haring and Domenico E. Pellegrini-Giampietro and Suzanne Zukin and R Suzanne Zukin and Marcel Scheideler}, journal={Molecular pharmacology}, year={1991}, volume={40 5}, pages={666-73} }