High-efficiency liquid chromatography on conventional columns and instrumentation by using temperature as a variable I. Experiments with 25 cm x 4.6 mm I.D., 5 microm ODS columns.

Abstract

High plate numbers were obtained in conventional LC by coupling columns and by using temperature to reduce the viscosity of the mobile phase. At 80 degrees C up to eight columns of 25 cm x 4.6 mm I.D. packed with 5 microm ODS particles could be coupled generating 180,000 effective plates while the pressure drop was only 350bar. For routine work, a set of… (More)

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@article{Lestremau2006HighefficiencyLC, title={High-efficiency liquid chromatography on conventional columns and instrumentation by using temperature as a variable I. Experiments with 25 cm x 4.6 mm I.D., 5 microm ODS columns.}, author={François Lestremau and Andrew Cooper and Roman Szucs and Frank S. David and Pat Sandra}, journal={Journal of chromatography. A}, year={2006}, volume={1109 2}, pages={191-6} }