High-efficiency liquid chromatographic separation utilizing long monolithic silica capillary columns.

@article{Miyamoto2008HighefficiencyLC,
  title={High-efficiency liquid chromatographic separation utilizing long monolithic silica capillary columns.},
  author={Kosuke Miyamoto and Takeshi Hara and Hiroshi Kobayashi and Hironobu Morisaka and Daisuke Tokuda and Kanta Horie and Kodai Koduki and Satoshi Makino and Oscar N{\'u}{\~n}ez and Chun Ming Yang and Takefumi Kawabe and Tohru Ikegami and Hirotaka Takubo and Yasushi Ishihama and Nobuo Tanaka},
  journal={Analytical chemistry},
  year={2008},
  volume={80 22},
  pages={8741-50}
}
Long monolithic silica-C18 capillary columns of 100 microm i.d. were prepared, and the efficiency was examined using reversed-phase HPLC under a pressure of up to 47 MPa. At linear velocities of 1-2 mm/s, 100,000-500,000 theoretical plates could be generated with a single column (90-440 cm in length) using an acetonitrile-water (80/20) mobile phase with a column dead time (t0) of 5-40 min. It was possible to prepare columns with a minimum plate height of 8.5 +/- 0.5 microm and permeability of… CONTINUE READING
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