High efficiency germ-line transformation of mosquitoes

  title={High efficiency germ-line transformation of mosquitoes},
  author={Neil F. Lobo and John Randall Clayton and Malcolm J. Fraser and Fotis C. Kafatos and Frank H. Collins},
  journal={Nature Protocols},
The ability to manipulate the mosquito genome through germ-line transformation provides us with a powerful tool for investigating gene structure and function. It is also a valuable method for the development of novel approaches to combating the spread of mosquito-vectored diseases. To date, germ-line transformation has been demonstrated in several mosquito species. Transgenes are introduced into pre-blastocyst mosquito embryos using microinjection techniques that take a few hours, and progeny… CONTINUE READING

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progress and prospects for RNAi-based approaches

  • A. E. Brown, Catteruccia, F. Toward silencing the burden of malaria
  • Biotechniques 40, Suppl: 38–44
  • 2006

what is the fitness cost? Trends Parasitol

  • M. T. Marrelli, C. K. Moreira, D. Kelly, L. Alphey, Jacobs-Lorena, M. Mosquito transgenesis
  • 22, 197–202
  • 2006

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