High efficiency gene transfer into the embryonic chicken CNS using B-subgroup retroviruses.

@article{Homburger1996HighEG,
  title={High efficiency gene transfer into the embryonic chicken CNS using B-subgroup retroviruses.},
  author={S A Homburger and Donna M Fekete},
  journal={Developmental dynamics : an official publication of the American Association of Anatomists},
  year={1996},
  volume={206 1},
  pages={
          112-20
        }
}
Attempts to use replication-competent retroviruses to target genes to the chick CNS have met with limited success for injections performed prior to stage 14 using A- or E-subgroup viruses. This study was aimed at improving CNS infection by varying the stage of injection, viral envelope subgroup, viral titer, and the presence or absence of a transgene and/or the polycation polybrene in the inoculum. RCASBP vectors were injected into the neural tube of stages 3-13 embryos and protein expression… CONTINUE READING
BETA

Citations

Publications citing this paper.
SHOWING 1-10 OF 20 CITATIONS

The RCAS retroviral expression system in the study of skeletal development.

  • Developmental dynamics : an official publication of the American Association of Anatomists
  • 2009

Versican in the developing brain: lamina-specific expression in interneuronal subsets and role in presynaptic maturation.

  • The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 2005
VIEW 3 EXCERPTS
CITES METHODS & BACKGROUND

Similar Papers

Loading similar papers…