High-efficiency formation of influenza virus transfectants.


cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 10(5) viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (10(6) cells) for RNP transfection. In addition to genes coding for surface proteins (hemagglutinin and… (More)


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@article{Enami1991HighefficiencyFO, title={High-efficiency formation of influenza virus transfectants.}, author={Miki Enami and P. Palese}, journal={Journal of virology}, year={1991}, volume={65 5}, pages={2711-3} }