High-efficiency cellular reprogramming with microfluidics

@article{Luni2016HighefficiencyCR,
  title={High-efficiency cellular reprogramming with microfluidics},
  author={Camilla Luni and Stefano Giulitti and Elena Serena and Luca Ferrari and Alessandro Zambon and Onelia Gagliano and Giovanni Giuseppe Giobbe and Federica Michielin and S Kn{\"o}bel and Andreas Bosio and Nicola Elvassore},
  journal={Nature Methods},
  year={2016},
  volume={13},
  pages={446-452}
}
We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-β and other signaling pathways exhibited temporal regulation that appears critical to acquisition of… 
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80 Citations
Highly Influential Citations
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Background Citations
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Methods Citations
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80 Citations

Microfluidic reprogramming to pluripotency of human somatic cells

A protocol to reprogram human somatic cells to hiPSCs with high efficiency in 15 d using microfluidics and enables hundreds of cells to be simultaneously reprogrammed, with an ~100-fold reduction in costs of raw materials compared to those for standard multiwell culture conditions.

Biophysical regulation of cell reprogramming.

Human Fibroblast Reprogramming to Naïve Induced Pluripotent Stem Cells in Microfluidics

The microfluidics approach will allow robust and cost-effective production of patient-specific niPSCs for regenerative medicine applications, including disease modelling and drug screening.

Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem Cells.

This approach does not require stable genetic modifications, is reproducible and cost-effective, allowing to produce patient-specific iPSCs for cell therapy, disease modeling, and in vitro developmental studies.

Direct generation of human naive induced pluripotent stem cells from somatic cells in microfluidics

It is reported that human naive iPSCs (niPSCs) can be generated directly from fewer than 1,000 primary human somatic cells, without requiring stable genetic manipulation, via the delivery of modified messenger RNAs using microfluidics.

Engineering Biomaterials with Micro/Nanotechnologies for Cell Reprogramming.

An overview of the state-of-the-art technologies for cell reprograming is presented and the recent breakthroughs in engineering biomaterials with micro/nanotechnologies to improve reprogramming efficiency and quality are highlighted.

MYOD modified mRNA drives direct on-chip programming of human pluripotent stem cells into skeletal myocytes.

Highly parallelized human embryonic stem cell differentiation to cardiac mesoderm in nanoliter chambers on a microfluidic chip

This study demonstrates that microfluidic technology can be used to automatically culture, differentiate and study hESC in very low-volume culture chambers even without continuous medium perfusion, an important step towards further automation and parallelization in stem cell technology.

Micropatterned substrates to promote and dissect reprogramming of human somatic cells

A micropatterned substrate is developed that allows for dynamic live-cell microscopy of thousands of cell subpopulations undergoing reprogramming and can be used in high-throughput to probe and understand the subcellular changes that accompany human cell fate transitions.

Timely delivery of cardiac mmRNAs in microfluidics enhances cardiogenic programming of human pluripotent stem cells

The combination of stage-specific regulation of Wnt/β-catenin signaling with mmRNAs encoding cardiac transcription factors will be suitable to obtain heart-on-chip model in a cost-effective manner, enabling to perform combinatorial, multiparametric, parallelized and high-throughput experiments on functional cardiomyocytes.
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