High-efficiency cellular reprogramming with microfluidics

  title={High-efficiency cellular reprogramming with microfluidics},
  author={Camilla Luni and Stefano Giulitti and Elena Serena and Luca Ferrari and Alessandro Zambon and Onelia Gagliano and Giovanni Giuseppe Giobbe and Federica Michielin and S Kn{\"o}bel and Andreas Bosio and Nicola Elvassore},
  journal={Nature Methods},
We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-β and other signaling pathways exhibited temporal regulation that appears critical to acquisition of… 

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